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In order to gain more material, 30 ng of normalised cDNA were used for 100 μl PCR and 7 cycles of PCR amplification with SMART PCR primer were performed as suggested by Evrogen (http://www.evrogen.com/kit-user-manuals/Trimmer-2.pdf).
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Simply revisiting the material may be enough for faculty to gain more out of the material.
Moreover, it is important to gain more experience since the material choice will always be based on the experience and preference of the surgeons.
Enterprises that focus on meeting emerging norms gain more time to experiment with materials, technologies, and processes.
He firmly believes in the steel industry's need to consolidate in order to gain more control over its pricing and raw material costs (see: "Bending Steel").
The group has also begun releasing sophisticated Internet material, in what appears to be a bid to gain more recruits.
We wanted to gain more leverage.
It will probably gain more this season.
Southwest will gain more market share.
Gain more patience.
As a result, we may gain more insights into how people learn from narrative information and more understanding about the use of narrative information in health education materials.
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CEO of Professional Science Editing for Scientists @ prosciediting.com