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For semi-quantitative imaging, control and mutant tissues were processed for immunofluorescence in parallel and images were acquired by confocal microscopy with the equivalent settings (laser power, gain, magnification and thickness of the stacks).
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First, all the FRET images were recorded using identical parameters, including excitation laser intensity, detector gains and magnification.
With a high-gain (low magnification) setting, participants can quickly maneuver the instrument jaw to the vicinity of the target (gross-positioning), but final acquisition of the target is exacerbated by the difficulty in precisely controlling the final position of the instrument jaw (fine positioning).
Treated and untreated animals were mounted on the same slide, and imaged with the same magnification, gain, offset, pinhole and laser power settings.
After snapping a series of photos at the ridiculous magnification gained through his reversed macro lens, he averages them together to get a crisp, clean, Christmas card-ready crystal.
This study evaluates the additional information gained from computerised magnification views in the mammographic workup of 100 women presenting with calcification on screening or follow-up mammography.
Low-gain settings (high magnification), on the other hand, facilitate fine positioning of the instrument jaw, but increase the time to advance the instrument jaw over large distances (gross-positioning) [ 62].
The same magnification and gain settings were used for all images.
As these cultures were grown at a lower density than the hippocampal cultures, we needed to capture 10 15 fields in time-lapse multi-positioning imaging at lower magnification to gain enough statistical power.
Laser gains, pinhole setting, and magnification was set identical across samples.
We imaged these spheres with exactly the same instrumentation parameters (camera gain and temperature, exposure time, magnification, etc).
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