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Furthermore, a pool of 105 peptides (15-mers overlapping by 11 amino acids (aa) encompassing the Gag protein (aa gag 1 - gag 432) of HIV-1 from consensus strain IIIB was used in ICS in order to evaluate the response to this protein by the CD4+ T-cell subset.
Most current review articles have settled on a value of 2500 Gag polyproteins and a ratio of 20 Gag : 1 GagPol, with some primary sources listed in Table 2. HIV-1_0.1.5 HIV-1_0.1.50 Gassumes 90 GagPol for a total of 1710 polyproteins.
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The number of you who have emailed in with the "Swiss defence like Swiss cheese - full of holes" gag: 897.
Serum His-Gag p41-specific antibody levels were found to be significantly higher at 1 and 0.5 μg doses of Gag p41-His Ni-NCs (His-Gag p41-His Ni-NCs) comp41-His Ni-NCs-Gag p41 (1 μg) adjuvanted with aluminum hydroxide (AH).
CMV-driven expression plasmids encoding HIV-1 Gag (pCR3.1-Gag) and HIV-1 Gag-mCherry (pCR3.1-Gag-mCherry) were kind gifts of Dr. Sanford Simons (Rockefeller University, New York, NY) and those that encode MLV Gag-EGFP (pcDNA3-MLV-Gag-EGFP) and SFV Gag-EGFP (pcDNA3-SFV-Gag-EGFP) were kind gifts of Dr. Stephan Gould (Johns Hopkins University, School of Medicine, Baltimore, MD).
The serum IgG2a levels induced by Gag p41-His Ni-NCs (1 μg) were significantly higher than AH adjuvanted His-Gag p41-His Ni-NCs
Responses to Gag p24 or to Pol were associated with lower proviral DNA load.
The optimal binding ratio of His-tagged Green Fluorescent Protein (His-GFP) and His-tagged HIV-1 Gag p41 (His-Gag p41) to the Ni-NCs was 1 221 and 1 480 w/w, respectively.
Mice immunized with the Gag(p17 -E2 60-mer scaffold particles mounted a strong and sustained antibody response.
In contrast, oral immunization with CVB4/p24 733) elicited both mucosal and systemic gag p24-specific T cell responses.
A consensus gag p24 protein sequence was generated and 147 of 148 codons were identical to CRF02-AG (IbNG).
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