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Four- to 6-week-old male nude mice (26 30 g) were injected subcutaneously in the hip with 200 μl of MKN45 cells.
One control animal (275 g) and two kainic acid-treated animals (medium and severe SE, 263 to 296 g) were injected i.v. with 37 MBq [18F]-PBR111 under isoflurane anaesthesia (5% induction, 1% to 3% maintenance in medical oxygen).
Hartley male guinea pigs (Charles River Laboratories, UK) at 3 weeks of age, weighing 250 to 330 g were injected intraperitoneally with 107 in vitro cultivated Leptospira in a final volume of 500 µl medium.
Female Fischer 344 rats (Taconic Farms Germantown, NY, 170 190 g) were injected via the tail vein (200 µl/rat) with a mixture of PA+LF (LT), prepared in sterile PBS.
Sexually mature, pre-spawning (mid-May; GSI 4.5±1.3%) female goldfish (15 40 g) were injected intraperitoneally with either SKF 38393 (D1 agonist; SKF; 1-phenyl-2,3,4,5-tetrahydro- 1H -3-benzazepine-7,8-diol; 40 µg/g) or LY 1-phenyl-2,3,4,5-tetrahydro- 1H -3-benzazepine-7,8-diol/g) 1-phenyl-2,3,4,5-tetrahydro- 1H -3-benzazepine-7,8-diol
To ensure that there was no non-specific binding of the anti-podocyte antibody in organs outside the kidney, male Balb/c mice (n = 4, bodyweight 22 35 g) were injected with a single dose (15 mg/20 g BW) of mouse anti-podocyte antibody into the tail vein.
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Glucose (5 g) was injected through the central venous line to calculate IDVG.
The disease group (Group G) was injected with 150 mg/kg/day GM (i.p).
Rats of Wistar or Sprague-Dawley strain weighing 150-200 g are injected subcutaneously with 100-175 mg/kg alloxan [ 44].
To calculate IDVG 10 ml of 50% glucose solution (5 g) was injected through the central venous line, as reported previously [ 1- 3].
DCM-S (3 g) was injected in a 46 × 3 cm column containing 200 g of silica gel and eluted with a step-gradient of hexane-methanol of increasing polarity.
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