This gene set, G, is filtered to only contain genes targeted in the siRNA screen.
The tags in the Webtag relational database were constructed as shown in Figure 2. Initially, all combinations of 5 bases long sequences containing the 4 bases A, T, C and G were filtered in order to select adequate "3'end regions".
Extracts (300 g) were filtered to obtain a fraction of crude alkaloids and then extracted with a ten-fold volume of 95%% (v/v) ethanol under reflux for 1 h.
In general, the digitized of Equation (1) is adopted, and it is written in matrix form as K F = G (2). where K is coefficient matrix, F and G are filtering vector and desired response vector which are digitized forms of F and G, respectively.
After the aqueous solution containing 50 g rye seeds was filtered and the seeds discarded, 50 ml V8 juice, 0.2 g CaCO3, and 17 g agar were added to the filtrate.
The supernatant was dialyzed against three changes of 2 L of loading buffer (150 mM NaCl, 25 mM CaCl2, 25 mM Tris Cl, pH 7.8), centrifuged for 5 min at 8000 × g and 30 min at 1,00,000 × g, the supernatant was filtered through glass wool and applied to a 10 mL column of mannose- or fucose-Sepharose (Fornstedt and Porath 1975).
The suspension was centrifuged for 10 minutes at 2,500 × g, the supernatant was filtered through two layers of gauze, and the mitochondria were sedimented at 7,700 × g for 10 minutes.
The white solid (5.3 g, 69 %) was filtered, washed with EtOH and dried in an oven at 60 °C.
As a result, it has been estimated that only about 2 g of albumin was filtered each day across the glomerular capillary wall.
After centrifugation at 15.000 g, the solution was filtered through a 0.45 µm filter and soluble Bet v 1.0401 was purified by hydrophobic interaction chromatography using a 100 ml Phenylsepharose column (GE Healthcare).
