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Hsiao (18 g) was extracted via circumfluence extraction with 60%% ethanol for 1.5 h, and was then filtered.
Powdered frozen leaf tissue (0.25 g) was extracted with 750 ml extraction buffer (50 mM Tris-HCl pH 8.5, 14 mM mercapto-ethanol, 50 g/l polyethylene glycol).
For cold water extraction, a sample (10 g) was extracted by stirring with 100 mL cold water at 4°C for 24 hours [ 3].
The crushed and dried S. polycystum (3.4 g) was extracted with methanol.
The crushed and dried material of S. ilicifolium (500 g) was extracted with methanol.
Minced raw snail (200 g) was extracted as described for the thermally processed samples above.
The crushed material of T. ornata (680 g) was extracted with methanol.
A test portion (50 g) was extracted with 200 ml methanol/water solvent solution containing 5 g of sodium chloride.
The powder (0.5 g) was extracted with 10 mL dichloromethane on a rotator for 1 h at 30 rpm.
The dried mycelia sample (2 g) was extracted with 50 mL of 95% ethanol for three times.
An accurately weighed sample powder (0.1 g) was extracted by ultrasonication with 7 mL 80 % methanol for 35 min.
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