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Glycogen productivity (g L−1 d−1) was estimated by dividing glycogen production by cultivation time.
Ethanol yield and ethanol productivity (g L−1 h−1) were calculated based on the mean of fermentative cycles.
Ethanol productivity (g L−1 h−1) was determined by computing the ratio of ethanol concentration to the fermentation time.
Specific ethanol productivity (g ethanol/g cell dry weight/h) was determined using the final OD660 measurement (91 hours) for each batch fermentation.
Enzyme productivity (g glucose produced/g enzyme applied) increased between 30 and 50% by the recycling, depending on the reaction conditions.
μ, specific growth rate (h-1); P, ethanol concentration produced (g L-1); Q p, volumetric ethanol productivity (g L-1 h-1).
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This modular design ensured high yield (0.96 g g−1), productivity (12.4 g L−1 h−1), concentration (250 g L−1) and purity (95%) in a very simple, environmentally benign, compact and flexible plant configuration reflecting all the major characteristics of high process intensification.
Fed-batch fermentation experiments revealed K. pneumoniae HSL4 exhibited an excellent ability to produce PDO with high concentration (80.08 g L−1), productivity (2.22 g L−1h−1) and conversion (0.435 g g−1 or 0.53 mol mol−1).
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