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Mice were sedated via intraperitoneal injection of ketamine/rompun (working concentration of 0.1 ml/10 g per mouse).
Considering a body weight of 25 g per mouse, clinical translation of iPSC-Mac for the treatment of respiratory infections would require a maximal cell dose of 1 × 1010 iPSC-Mac for a complete lung of a 60 kg patient.
The average weight of the mice was 15 g per mouse.
There was no difference in feed intake (3.4 ± 0.04 g per mouse and day) and final body weight (23.6 ± 0.4 g) between groups as expected on an isocaloric diet.
Therefore, the intake of cadmium in the high group was considered as 1.95 ± 0.24 g per mouse daily and in the low group was considered as 0.975 ± 0.12 g per mouse daily.
Seven-week-old female Swiss-Webster mice (weight ~25 g per mouse) were purchased from the Dalian Medical University in Dalian, China (SCXK 2008-0002).
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After three feedings, 1 week apart, 1 g per mice, no differences were detected in the growth rate or behavior of the animals fed with these three types of spinach.
To determine the CFU per g feces, mice were placed in individual cages with no bedding for 30 40 min. After this time, mice were returned to their original cage and fecal pellets were collected, weighed, and resuspended 1 10 w/v in PBS.
It was calculated that approximately 36 g α-TEA and 0.4 g 9-NC per mouse per day were deposited in the lungs, respectively.
Tamoxifen (Sigma-Aldrich, St Louis, MO) was dissolved at 20 mg/ml in corn oil and injected intraperitoneally at 9 mg per 40 g mouse 3 times at 2-day intervals [36].
When the activity of myostatin and other activin receptor type IIB ligands was reduced by systemic administration of a soluble activin receptor type IIB for 10 weeks, there was an increase in insulin-stimulated glucose uptake per g muscle when mice were on a normal diet, but not when they were on a high-fat diet [9].
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