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Cobweb extract was prepared through hydrolysis of 0.1 g of washed and air-dried cobweb with 10 ml of 0.1 M NaOH at 90 °C for 1 h.
Approximately 12 g of washed or unwashed WIS and Tween 40 according to a certain ratio of substrate to Tween 40 were added to 250 mL Erlenmeyer flasks.
High-solids enzymatic hydrolysis experiments were performed in 125 mL wide-mouth polypropylene bottles (Thermo Fisher Scientific, Inc., Waltham, MA) loaded with 60 g of washed slurry at 20% insoluble solids.
The α-L-arabinofuranosidase production was carried out in 250-ml Erlenmeyer flasks containing 5 g of washed dried agro residues (wheat bran, wheat straw, rice bran, rice straw, corn cobs, and maize bran) moistened with 10 ml of modified Mandels-Weber medium containing the following (g/l): urea, 0.3; ammonium sulfate, 1.4; KH2PO4, 0.3; CaCl2, 0.3; MgSO4.7H2O, 0.3; proteose peptone.
Each glass vessel contained 500 g of washed aquarium sand (diameter 1 2 mm) at the bottom, which served as a support for bacteria to promote self-purification of the test system.
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(4.3 g) of washing soda and stir it.
(4.3 g) of washing soda at a time and stirring until it will no longer dissolve.
If you use 1 cup (237 ml) of water, you will need 2 to 3 cups (412 to 618 g) of washing soda.
Table 2 shows the fermentation products of strain Caldicellulosiruptor DIB 004C upon growth on medium with 2.9 g/l of washed pretreated poplar.
At 2.9 g/l of washed pretreated poplar, the medium contained 10.4 mM glucose, 0.02 mM xylose, and 0.03 mM galactose equivalents.
In the current study, 0.1 g of acid washed pyrite was added to lipid solution (20 ml).
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