Exact(6)
In all cases, the metallic loading was referred to as 100 g of support.
50 mL of demonized water was added to a beaker containing 1 g of support.
The best APS for cyt c adsorption (in terms of capacity) was 30 nm, with loadings of 180 mg of protein (g of support)−1.
The precursor salt was dissolved in a volume of distilled water equal to the support pore volume (0.6 cm3 g−1 for alumina and 1 cm3 g−1 for silica) using 100 g of support.
One unit of immobilized lipase activity was defined as the amount of enzyme that released 1 mol of pNP per minute and per g of support, under the assay conditions used [ 21, 38].
Crude protein extracts were diluted 10 fold in 10 mM sodium phosphate at pH 7 and incubated at 80°C and pH 7 for 45 min. Protein aggregates were discarded after centrifugation (10,000 × g for 10 min), and the clarified supernatant containing the NOX activity was offered to different chromatographic supports (PEI-ag and SD-ag) at pH 7 and 25°C (1 g of support per 10 mL of protein extracts).
Similar(54)
In a typical experiment, the reaction mixture consists of 0.3 0.4 g of supported gold catalyst, 5 mL of cyclohexane (CH), 25 mL of acetonitrile as solvent, and 0.1 g oftert-butyl hydroperoxide (TBHP), unless otherwise stated.
In 15 mL Felkin tubes, 0.3 and 0.6 g of supports of HEC and chitosan, respectively, were added to a solution of Ag NP (10 mL) at a well-defined concentration and stirred with magnetic stirring bar for 2 h.
Using quantitative polymerase chain reaction (after reverse transcription) (qRT-PCR) analysis we found only a marginal upregulation of Cyp2b19 and PPARβ/δ expression in P2.5 and P32 K5cre-CMVcaNrf2 transgenic mice (Fig S1F and G of Supporting information).
Once cooled, the supports were ground and sieved to a particle size of 125 250 μm. 3 g of each support was added to 50 ml of a solution containing ammonium heptamolybdate tetrahydrate (Sigma-Aldrich, ACS, ≥99.99 %) cobalt (II) nitrate hexahydrate (Sigma-Aldrich, ACS, ≥98 %), nitric acid (Sigma-Aldrich, 70%%) and an excess of citric acid (Alfa Aesar, Anhydrous ACS, 99.5 + %) and distilled water.
An amount of 0.55 g of HNTs support was then added to this clear solution (5% Au loading), and the temperature of the resulting slurry was increased gradually to 90°C.
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