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10 g of sodium tungstate and 2.5 g of sodium molybdate were dissolved in 80 ml of water, and then 5 ml of conc.
Simultaneously, 60 g of sodium hydroxide dissolved in 700 ml of water in an another beaker, followed by mixing with 100 g of sodium aluminate.
Batches up to 500 g of sodium borohydride powder were hydrolyzed with liquid water.
The conditions determined as ideal were 0.1 g of sodium sulfate, 100 μL of toluene and 5 mL of water.
Finally, carbonate supporting buffer (0.1 M in each constituent) was prepared by dissolving 10.6 g of sodium carbonate (BDH, UK) and 8.4 g of sodium hydrogen carbonate (Winlab, UK) in one liter distilled water.
A sodium hydroxide solution was prepared by dissolving 10 g of sodium hydroxide into 100 mL RO water.
Then, 2.7 g of sodium acetate and 0.75 g of polyethylene glycol were added with constant stirring for 30 min.
Then, 2 g of trimethylamine hydrochloride and 1 g of sodium hydroxide were added to the mixture solution.
A test portion (50 g) was extracted with 200 ml methanol/water solvent solution containing 5 g of sodium chloride.
Then, 3.7 g of sodium bicarbonate was added into the flask and 10%% FBS was added and mixed well.
The best extraction conditions were achieved at 59 °C, with 60 min of extraction time and 2.91 g of sodium chloride.
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