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During SSF, 1 g of samples of the media were taken at different time points for analysis [16, 17].
For hydrogen storage measurements, 2 g of samples were loaded in a metal-hydrogen high pressure reaction chamber.
For sediment samples, up to 3 g of samples were stirred for 30 min in 100 ml of sterile Ringer's solution (5% v/v).
For soil and dust, 15 30 g of samples were taken from 3 to 5 cm depth of the sampling points and transferred to the laboratory.
The oils of the whole berries, pulp/peel and seeds were extracted from 5 g of samples using a methanol/chloroform extraction procedure [17, 32].
Figure 4 XRD patterns (a) and SEM images (b, c, d, e, f, g) of samples collected at different reaction times.
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About 2.5 g of sample was used in each measurement.
Per 0.2 g of sample, 3 mL of F12 K (Gibco®, Life Technologies, USA) was used.
Briefly, to 1 g of sample in a beaker added 5 mL of conc.
0.2 g of sample is sufficient to obtain accurate results with this type of analyser.
About 0.4 1 g of sample analyzed using mercury intrusion porosimetry following the German standard DIN 66133.
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