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About 2.5 g of sample was used in each measurement.
Every 5.0 g of sample was immersed in about 60.0 mL cow urine.
Briefly, to 1 g of sample in a beaker added 5 mL of conc.
0.2 g of sample is sufficient to obtain accurate results with this type of analyser.
The total phenolic content was expressed as mg gallic acid equivalent/100 g of sample.
Per 0.2 g of sample, 3 mL of F12 K (Gibco®, Life Technologies, USA) was used.
The measurement began by placing 0.1 g of sample powder on a Plexiglas plate.
The optimal extraction conditions were: 5 g of sample, 15 mL of acetonitrile and 3 min of agitation.
It is found that the E g of sample C and D is about 1.22 and 1.19 eV, respectively.
Weight of about 0.2 g of sample was transferred in a 50 ml of glass centrifuge tube.
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A large excess of Trizol (15 ml for 0.5 1.5 g. of sample) was used in order to prevent RNA degradation by endogenous RNAse.
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