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For the preparation of solid media, 10 g of medium were mixed with 25 mL of deionized H2O in a 250 mL Erlenmeyer flask, sealed with a foam plug and tinfoil, and autoclaved.
Subjects were supplemented with 56 g of medium chain triglycerides (MCTs).
Solid state fermentation for the production of cellulolytic enzymes (cellulolytic complex) was carried out in Erlenmeyer flasks of 250 mL with 10 g of medium culture, 0.5 g filter paper, and 1.0 mL of spores suspension (inoculum), containing 10 spores·mL−10
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A mixture of eight different N. whitei isolates (to ensure sufficient standing genetic variation in the parasite population) was used to infect the first generation of beetles in the coevolution treatment, by mixing spores into the standard medium at a concentration of 2 * 104 spores g-1 of medium.
The fabrication of nanofibers was achieved by electrospinning: a solution containing 1 g of PVP (medium molecular weight = 1,300,000 Da), and 0.36 g of zinc acetate (Zn(CH3COO 2 · H2O) in 2 ml of dimethylformamide (DMF) (all reagents were purchased from Sigma Aldrich), was added to 1 ml of Pt nanoparticles solution, in order to obtain a solution with 1 % of Pt nanoparticles.
Fermentations were carried out in 300 mL Erlenmeyer's flasks containing 50 g of the medium, which were incubated at 30°C for 96 h after inoculation.
Briefly, for rice (japonica rice; round and short grain), participants selected from nine options ranging from <1 bowl per day to ⩾10 bowls per day and rice-bowl portion size: small (110 g of rice), medium (140 g of rice) and large (170 g of rice).
One end of the tube was inserted in 5 g of culture medium kept in a plastic container (vol. 10 ml) which served as the source of food and water for the fly.
The extraction of lipase from the fermented bran obtained in solid-state fermentation by the fungi Aspergillus niger was carried out by adding 10 mL buffer with pH established in each methodology at 1 g of fermented medium, followed by agitation of 160 min−1 for 30 min at 37°C.
Culture medium for the HGFs was prepared using 5 g of minimum essential medium (Alpha medium) from GIBCO™ (Cat. no. 12000 041, Lot no.
The two groups had similar representations of low-density (≤100 mites per g of dust), medium-density (≤100 mites per g of dust), and high-density (>500 mites per g of dust) samples, as well as similar densities overall (Table 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com