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Hence, for the sake of economic feasibility, 8 g of inoculum size was selected for further studies.
The samples consisted of 450 g of inoculum and 2.5 g of VS of the substrate, each in three replications.
A minor difference in decolorization potential in case of 12 and 8 g of inoculum size was observed.
The samples consisted of 500 g of inoculum and approximately 2.5 g of volatile solids (VS) of the substrate, each in three replications.
Soil infestation was carried out 7 days before planting by mixing 180 g of inoculum to the soil in every pot and mixed thoroughly to ensure equal distribution of fungal propagates, followed by irrigation.
The remaining mesocosms received 500 ml of filtrate from 500 g of inoculum (with no mycorrhizal spores) and 500 g sterilized inoculum to correct for possible differences between the microbial communities in mycorrhizal and non-mycorrhizal treatments.
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OLR was expressed in g of TCOD fed per kg of inoculum per day, and g of total VS fed per kg inoculum per day.
For the model validation, it was attained 0.60 U mg−1 with 70 g L−1 xylose, pH 7.5 and 1 g L−1 of inoculum level.
We fed (in muesli balls, monitored by video to ensure that the total amount was eaten) 5 g of BSE inoculum to each of 12 macaques (macaques S1 S5 and S9 S15), 8 16 g of BSE inoculum to each of 6 macaques (macaques S6 S8 and C1 C3), and mock inoculum (non-BSE infected brainon-BSE infectedach of 8 macaques (macaques M1–M8).
A maximum yield of 565.76 ml CH4/g VSadded was estimated under the optimum conditions for substrate concentration 7.77 g of VS/l, inoculum to substrate ratio of 2.81 and calcium concentration of 380.82 mg/l.
In +AMF treatments, microsites additionally contained 25 g of AMF inoculum.
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