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Fermentation of FOW was carried out in 1 l Erlenmeyer flasks, each containing 400 g of fermentation mixture.
After the fluid was mixed 2 1 with McDougall's buffer, 150 mL of inoculum was added to the fermentation flasks (n = 4/heifer) with 2.5 g of fermentation substrate.
In experiment 1, we combined strained and pooled rumen fluid from 3 heifers in a 2 1 ratio with McDougall's buffer, and added 150 mL of the inoculum to each flask (n = 5/treatment) with 2.5 g of fermentation substrate similar to a lactating cow ration, ground to 1 mm.
The musts were brought to 20 °C room temperature for the start of fermentation; 5 g of fermentation nutrient (Fermaid E, Lallemand, Vienna, Austria) were added to each demijohn.
When the wines reached 8% (v/v) alcohol, a further 2 g of fermentation nutrient (Fermaid E) were added per demijohn and the fermentations were completed at 20 °C.
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For the metagenome, aliquots of 20 g of the fermentation sample were used for total community DNA preparation as described previously [ 7].
This method produced high yields (1.88 g/L of fermentation broth) of protein, which could be purified to homogeneity.
Sufficient active dried yeast (0.5 g/L of fermentation medium) was rehydrated with the goal of providing a starting concentration of ~107 viable cells/ml in the fermentation medium.
The lipase activities in volume were associated with simultaneous changes in corn starch concentration (8, 10, and 12 g/L), the soybean meal concentration (30, 35, and 40 g/L), and the soybean oil concentration (5, 10, and 15 g/L) of fermentation medium.
One maker mentioned needing 350 400 g of yeast for fermentation during cold weather.
During the 34 h of fermentation, 3.8 g l−1 of xylose and 2.5 g l−1 of arabinose were consumed with 12.6 g l−1 of lactic acid, 4.8 g l−1 of acetic acid and 4.0 g l−1 of ethanol produced.
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