One kilogram bottom ash is able to sequestrate more than 3.0 g of hydrogen sulphide, 44 mg of methyl mercaptan, and 86 mg of dimethyl sulphide.
Next, 8.5 g of erucylamide dimethyl amine was dissolved in 50 ml acetone and then poured into the flask.
After cooling to room temperature, 6 ml of absolute ethanol was added and mixed gently. 1 ml of Ehrlich reagent (1.6 g of N N dimethyl-p-aminobenzaldehyde in 60 ml solution containing 50 50 (v/v) of absolute ethanol: concentrated HCl) was added and mixed gently.
A mixture of 0.02 g of 4-dimethyl-aminopyridine, 0.9 mL of pyridine, and 0.496 g of glycerol 32 was added dropwise to the cooled, stirred acid chloride solution.
A mixture of 0.02 g of 4-dimethyl-aminopyridine, 0.5 mL of pyridine, and 5 mL of 1,2-dihydroxyethane 27 were added dropwise to the cooled, stirred acid chloride solution.
Compound (1) is 13,13'- 2,5-dibromo-1,4-phenylene bis(oxy)bis(2,5,8,13,13'- 2,5-dibromo-1,4-phenylene bisd by incubating at 80°C overnight a suspension of 2-{2-[2-(2-methoxyethoxy)ethoxy]ethoxy}thyl 4-methylbenzenesulfonate13,13'- 2,5-dibromo-1,4-phenylene bisuinone (13,13'- 2,5-dibromo-1,4-phenylene bis13,13'- 2,5-dibromo-1,4-phenylene bis(DMF).
After cooling down to room temperature, 6 ml of absolute ethanol was added and then mixed gently. 1 ml of Ehrlich reagent (1.6 g of N-N dimethyl- p-aminobenzaldehyde was added into 60 ml solution containing 50 50 (v/v) of absolute ethanol: concentrated HCl) was added.
For the cytotoxicity and antiviral assays, a stock solution was prepared by dissolving 2.0 g of extract in 100 mL of dimethyl sulfoxide (DMSO) (Sigma Aldrich, USA).
Original concentration was made by dissolving 9 g of extracting plant material in 5 ml of dimethyl sulphoxide (DMSO 99.5%) to prepare a final volume of 15 ml.
The dialyzed product in 0.1 M Hepes buffer (pH 8.8) was reacted with diethylene triamine pentaacetic acid anhydride (DTPA, 0.5 g, 1.4 mmol; Sigma) suspended in 2.5 ml of dimethyl sulfoxide (DMSO) at room temperature.
