Sentence examples for g of cold from inspiring English sources

Pellets were resuspended in 1.5 mL lysis buffer plus 0.2 U/µl RNasin (Promega) per gram of cell pellet, and then 5 g of cold 0.5 mm glass beads per gram of cell pellet was added.

To prepare ESC-extracts, cells were washed with PBS once followed by one wash with cell lysis buffer (100 mM HEPES, pH 8.2, 50 mM NaCl, 5 mM MgCl2, 1 mM dithiothreitol, and protease inhibitors), followed by sedimentation at 400 g, suspension in 1 volume of cold cell lysis buffer, and incubated for 30 45 min on ice.

Sample (2.0 g) was mixed with 20 ml of cold 40 mM phosphate buffer (pH 6.8), and the mixture was homogenized at 13,500 rpm for 10 s.

For nucleic acid (DNA and RNA) determination, the plant tissues (0.25 g) were homogenized in 2 mL of cold methanol, and insoluble pellet was washed twice with same volume of methanol.

The prepared hepatopancreas (20 g) was homogenized with 90 ml of cold solvent mixtures (isopropanol: hexane, 50: 50, v/v) (4 °C) at the speed of 9500 rpm using an IKA Labortechnik homogenizer (Selangor, Malaysia) for 2 min at 4 °C.

Briefly, nuclei were resuspended in lysis buffer (Tris HCl 50 mM pH 8, MgCl2 5 mM, KCl 25 mM containing HCl 0.2 N, 1 mM NaF, 1 mM Na3VO4, 2.5 mM sodium butyrate and Roche's EDTA-free protease inhibitor cocktail), and incubated on ice for 30 min. After centrifugation at 11,000 g for 15 min, 1 ml of cold acetone was added to the supernatant and placed at −20°C for 4 h.

Briefly one tablespoon of ground bark (8.3980 g) was added to 200 mL of cold water and "shaken" seven times.

Yeast cells (about 50 mg dry mass or 10 cells) were collected by centrifugation (5 min, 3000 g), washed once with 10 mL of cold sterilized water, and after a second centrifugation, cell pellet was resuspended in cold water.

For the parasite fractions, cells were washed twice in 10 ml of cold PBS (800 g 10 min, 4°C) and resuspended in 400 µl of lysis buffer containing protease inhibitors (as described above) on ice.

One maker mentioned needing 350 400 g of yeast for fermentation during cold weather.

For biochemical studies 0.25 g brain tissue was homogenized in 3 ml of cold saline.