A total 706.68 g/L ABE (54.2 g/L of ethanol, 169.93 g/L of acetone, and 482.55 g/L of butanol) was collected after 11 h of pervaporation separation.
After the titration of lead, zinc was quantitatively de-masked from its cyanide complex by the addition of (3:1) 20 g of 4% formaldehyde:acetone solution.
Samples were concentrated under reduced pressure to scale down the volume, and aliquots of 10 μL were applied on TLC plates (Merck, Berlin, Germany), run in butanol : methanol : ammonia : water (5 : 4 : 2 : 1, v/v/v/v) and stained using aniline-diphenylamine reagent (4 mL of aniline, 4 g of diphenylamine, 200 mL of acetone, and 30 mL of 85% phosphoric acid).
The extraction parameters were optimized using the response surface method based on central composite design: 791 μL of acetone solvent, 2.5 g of Na2SO4, pH 1.7, 3.0 min of stir time, and 5.5 min centrifugation.
Fresh leaves and green and red peppers (2 g) were extracted with 20 mL of acetone, followed by homogenization with an Ultra-Turrax T 25 (IKA, Staufen, Germany) for 30 s at 17,500 rpm.
A part of acetone extract (70 g) was dissolved and fractionated in a mixture (1/1, v/v) of chloroform and water to yield the water and chloroform fractions.
The powder (0.1 g) was suspended in a solution of acetone and 50 mM citric acid (70 30, v/v) and [9,10-H2]-9,10-dihydro-JA (20 ng) was added as an internal standard.
Methyl 3,12-Dioxo-18βH-olean-9 3,12-Dioxo-18βH-olean-9 3,12-Dioxo-18βH-olean-9 3,12-Dioxo-18βH-olean-9 3,12-Dioxo-18βH-olean-9 (33 %)[ 45] was added dropwise to a solution of 7 (5.11 -en-30-oatene (500 mL) at 0°C over 30 min till the brown color persisted.
