Surface soils and plants samples (each weighing about 500 g) were taken in triplicate, at the same distance from the street across a 1x1 m2 area by employing a stainless steel trowel to a 20 cm depth from the surface.
aos and wild-type Col-gl plants weighing approximately 1 g were harvested in triplicate, ground in liquid nitrogen, and the powder was resuspended in 5 ml methanol and incubated at 4°C overnight.
For the recovery experiments, pesticide-free samples (20 g) were spiked in triplicate (n = 3), after homogenization by the addition of appropriate volumes of pesticides standards at two different levels (0.05 and 0.50 μg/mL).
The enzyme mixtures were dissolved in 1 ml of citrate phosphate buffer (pH 6.0) and sprayed on 0.5 g of bahiagrass hay in triplicate in each of two runs.
Biomass (2.0 g DM) was pretreated in triplicate under the conditions shown in Table 1.
For each foam sample G' and G" measurements were performed in triplicate at 23 °C.
For the comparison of ASE, ultrasonication, and shaking with chloroform, 1-g samples were taken in triplicate from five Dutch peppers of one Middenmeer greenhouse batch (cultivar Tamarin) that were freeze-dried, milled, and combined.
Samples weighing 2 g were dried in triplicates an oven at 105 °C for 3 h.
Each soil sample (100 g) was obtained in triplicates from the upper 10 cm of soil depth and mixed well in sterile polyethylene bags.
Briefly, a.5 g goji berry sample in triplicates was extracted with 5 mL of ethanolic butylated hydroxyl toluene (ethanol/BHT 100 1, v/w) for isolation and the release of carotenoids.
After the fertilizer, nitrapyrin and NBPT were supplied, the destructive sampling of ~20 g soil was conducted in triplicates at day 1, 3, 7, 14, 21 and 28.
