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(g) Detection of biotinylated recombinant GDF11 with streptavidin-HRP (left) or Coomassie staining (right).
Thus, this recombinant immunoconjugate is a promising marker for Toxoplasma serodiagnosis, requiring further evaluation on a larger series and could provide the basis for direct antibody capture enzyme-immunoassay for specific immunoglobulin M and G detection.
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PB-NKP and control cell lines were used: Jurkat cells stimulated for 30 min with IL-4 at 10 ng/ml for pSTAT-6 and GATA3, HL-60 cell line for IL-21 and FON melanoma cell line for HLA-G detection.
8-oxo-G detection was performed exactly as above for microscopic analysis.
For 8-oxo-G detection, cells were fixed in 4% paraformaldehyde in PBS and postfixed in 70, 95 and 99% methanol for 30 min at −20 °C and then rehydrated by 95 and 70% methanol at −20 °C for 3 min and three washes in PBS.
A small proportion of samples weighing < 2 g had detection limits that were higher than the usual detection limits.
In this paper, a simple fiber biosensor (FOB) for immunoglobulin G (IgG) detection is designed and experimentally verified.
After washing with PBS, immunoglobulin G (IgG) detection was performed with the Vectastain Elite ABC kit (Vector Laboratories, Peterborough, UK) according to the manufacturer's recommendations.
The antisense cRNA probe binds in situ to Gd-mRNA and was utilized for Gd-mRNA detection.
Lack of 10-g monofilament detection is generally associated with clinical disease that is predictive of future foot ulcers (15).
Diabetic men had less 10- and 1.4-g monofilament detection, higher average threshold vibration, lower CMAP, and lower NCV than nondiabetic men (Table 2).
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