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The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.
Further assessment of spatial morphology features will be a subject of future assay development.
These proteins can be regarded as "back-up" proteins in case a future assay for a protein within the diagnostic signature will not fulfill the required analytical criteria.
Finally, valuable recommendations could be derived that should guide the future assay harmonization efforts spearheaded by the CVC-CRI as well as other consortia active in this domain.
A recently developed assay system, that uses tandem mass spectrometry, has been reported to have extremely high specificity for steroid assays and might be considered for a future assay system [ 30, 31].
Objectives: The goal of this study was to develop methods to evaluate the data generated from these assays to guide future assay selection and prioritization for the Tox21 program.
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This system allowed a live inoculum stock to be directly used in all future assays.
This may affect the design of future assays and standardization approaches.
The paper gives indications as to what effect nucleotide changes at different positions within primers and probes might do and should aid in the testing of future assays, although it is difficult to draw fixed rules about the possible effect changes may have.
Several unstained sections both at 4 µm and 8 µm were collected on positively charged slides for future assays.
In future assays, it may be useful to grow worms and conduct rapid upshifts in a room maintained at 15°C by use of a temperature-controlled microscope stage, in order to bypass mounting embryos at room temperature (which would likely allow one to further clarify the "Unclear" determinations in Table 4).
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