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Treatment was concluded when the ring-apophysis fusion was complete on a LL X-ray [ 9].
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A few FCs fused each other in late G1 phase when the process of nucleoli fusion was completed.
GTXαΔ [Hin dIII/Bam HI] was then inserted into pPGK, and the GTXα fusion was completed by inserting the 3'-terminal Bgl II/Bam HI fragment of Xα into the Bam HI digested vector pGTXαΔ.
PET-MRI fusions could be performed in all cases with no obvious limitations, even though fine manual readjustments had to be done in some cases after automatic fusion was completed.
ELISA analysis demonstrated positive hybridomas of anti-ERGIC3 mAbs after cell fusion was completed (a) and the positive rate of approximately 95% after rounds of sub-cloning was performed by limiting dilution (b).
Normally, Golgi tubulation and fusion are complete within 5 10 min of BFA addition.
The building blocks of Fusion were completed last year, says John Wookey, Oracle's applications chief, and by 2008 they will become a seamless amalgam of databases, applications and the middleware that stitches them together.
The process of palatal fusion is completed by week 12 in humans (E16 in mice) and is marked by the complete disappearance of the midline edge seam.
Four fusions were completed isolating 11 high affinity mAbs, two are isotype IgM and the remaining 9 are subisotype IgG1.
After overnight digestion the cleavage of the fusion protein was complete and SDS-PAGE analysis showed 2 bands (Fig. 4, lane 2), corresponding to the native DI (~8 kDa) and the fusion tag (~6 kDa).
Construction of the expression vector for the maltose binding protein (MBP −D108N AHL lactonase fusion protein was completed by using the overlap extension PCR method (35).
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