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On the other hand, no ookinates were detected in –HG- and –TM/C-KI parasites, suggesting that the highly conserved N-terminal domain and TM of PbGCS1 are similarly critical to parasite gamete fusion (Figures 6C and 6D).
This result indicates that the homologous recombination itself does not affect the male fertility and that the C-terminus of PbGCS1 less contributes to gamete fusion (Figures 6C and 6D).
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The monster in the "Frankenstein" series, for instance, is what Mr. Carroll called a "fusion figure," made of spare parts, including different brains.
The multilevel fusion approach can be regarded as an extension to decision fusion (Figure 4).
We also observed defects in lateral trunk (LT) fusion (Figure 6F, M).
An NKX2.2 mutant construct was developed containing only the TN and HD domains (TN-HD fusion; Figure 2A).
A carboxyl-terminal truncation to amino acid 240, which removes SIM1, greatly reduced modification of the VKPE fusion (Figure 2C).
We successfully captured the native endogenous RasGAP protein from the extract using the GST-RG27 fusion but not a GST-control aptamer fusion (Figure 4B).
We next evaluated the impact of increasing HA NA plasmid ratios (w/w) on the rates of cell-cell fusion (Figure 3).
We speculate that mitochondrial fusion (Figure 2) may also be involved in the reduction of oxygen consumption and, especially, on the H2O2 production observed at 24 h ABM.
When this plasmid was mobilised into J463 (a RifR mutant of the null strain ATCC17025) containing the acuI-lacZ fusion plasmid pBIO1915, this restored the ability of DMSP to co-induce this fusion (Figure 3E), consistent with this model.
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