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The monster in the "Frankenstein" series, for instance, is what Mr. Carroll called a "fusion figure," made of spare parts, including different brains.
The multilevel fusion approach can be regarded as an extension to decision fusion (Figure 4).
We also observed defects in lateral trunk (LT) fusion (Figure 6F, M).
An NKX2.2 mutant construct was developed containing only the TN and HD domains (TN-HD fusion; Figure 2A).
A carboxyl-terminal truncation to amino acid 240, which removes SIM1, greatly reduced modification of the VKPE fusion (Figure 2C).
We next evaluated the impact of increasing HA NA plasmid ratios (w/w) on the rates of cell-cell fusion (Figure 3).
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This result indicates that the homologous recombination itself does not affect the male fertility and that the C-terminus of PbGCS1 less contributes to gamete fusion (Figures 6C and 6D).
On the other hand, no ookinates were detected in –HG- and –TM/C-KI parasites, suggesting that the highly conserved N-terminal domain and TM of PbGCS1 are similarly critical to parasite gamete fusion (Figures 6C and 6D).
Amplified products were analyzed by direct sequencing to verify the presence of the different fusions (Figure 1C).
The total number of colonies was recorded 10 days post-fusion (Figure 3C).
As time progressed, the reprogrammed MEFs gradually obtained the morphology of ESCs, as reflected by comparisons of forward and side scatter profiles of the GFP-positive MEFs at 24, 48 and 72 h post-fusion (Figure 1B).
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