The fusion entailed very few personnel changes.
DOI: http://dx.doi.org/10.7554/eLife.01879.006 The vacuole fusion pathway entails early ATP-dependent reactions that occur on separate vacuoles, termed priming, followed by tethering, which can be mediated by HOPS and Ypt7p alone (Hickey and Wickner, 2010).
A full integration, or "fusion," would entail a single "generative" model that would explain both types of data [Aubert and Costalat, 2002; Riera et al., 2006; Sotero and Trujillo-Barreto, 2008; Trujillo-Barreto et al., 2001].
One way to assess the functions of DNA elements entails their fusion to fluorescent reporter genes.
Overall, this hypothesis does not alter the topology, but it does require one additional instance of chromosomal rearrangement, entailing one fusion followed by two independent fissions, thus it is less parsimonious than the inference of two independent fusions within both the melanica and robusta species groups.
If A1 entails increased heterotypic fusions without lowering homotypic fusions, both C-values increase continuously with the establishment of A1.
This technique entails expressing GST fusion proteins, allowing them to bind the target protein and purifying the complex by GST-glutathione affinity purification.
Such arrangement is often found in mitochondrial introns of Fungi, and it entails synthesis of a fusion protein encoded by the intron and the preceding exon(s) [ 32, 106, 107].
This approach entailed covalently conjugating the fusion protein TfRscFv-GAL4 to the plasmid via a GAL4 at the 3'-end of the protein recognizing GAL4rec on the transfected plasmid (GAL4rec-GFP-pGes).
Sexual reproduction entails, at a minimum, the fusion of two haploid cells to form a diploid cell that subsequently undergoes meiosis to produce four haploid progeny.
