This was particularly common in fusion proteins involving EWSR1, FUS and TAF15, since 48% of such fusions had incompatible reading frames that were corrected by one of these mechanisms (24 out of 50 gene fusions analyzed for these genes).
Interestingly, the final reading frame of the 5' exon and the starting reading frame of the 3' exon were compatible in 540 of the fusions analyzed, thus confirming that an in-frame fusion protein was generated in 93% of the cases.
In 43 of the 583 gene fusions analyzed, the reading frame of both exons seemed incompatible with an in-frame fusion product, so we went back to the original sequence to check whether other mechanisms had restored the reading frame in the fusion transcript.
Since most of the fusion sequences analyzed are derived from fusion transcripts (spliced mRNAs), these sequences already take into account potential exon skipping or alternative splicing events.
Lumen fusion was analyzed in γCOP10 homozygous embryos and heterozygous siblings by scoring the number of successful fusion events per ten tracheal metameres in stage 15 embryos stained for Chitin.
Performance results are given in Section 7, and the concluding remarks are given in Section 8. Distributed detection and decision fusion are analyzed by many authors in different contexts, for example, [1 6, 8 10], to name a few.
In order to determine the thermal stability of the microPCM, the enthalpy of fusion was analyzed after samples [i.e., the base case (PCM0.6)] were subjected to 50 thermal cycles at the same temperature range and heating rate.
In a second approach, FCP-lysosome fusion was analyzed by using LysoTracker red which selectively labels acidic lysosomes.
Nestin-GFP fusion proteins analyzed in 30 hpf embryos became undetectable after nestin MO treatment (Fig. 1F).
Cell fusion was analyzed in the chronically inflamed colon from male IL-10−/− mice that were transplanted with GFP-expressing WBM from a female donor.
