Many columns with fused core technology were also evaluated to attain resolution between LS and LHA, but due to high back pressure, these columns could not be used.
Current trends in fast liquid chromatographic separations involve monolith technology, fused core columns, high temperature liquid chromatography and ultra-high performance liquid chromatography (UHPLC).
The high efficiency and relatively low back pressure of these 2.7 μm Fused-Core particles has been maintained so that separations can be performed with conventional HPLC instruments.
The RP-HPLC separation in a fused-core column was also optimized allowing the separation of the three peptides extracted from maize kernels in 6 min.
The advantages of these new 400 Å fused-core particles, specifically designed for protein analysis, over traditional particles for protein separations are demonstrated.
This report describes the effects of fused-core particle size, pore size, shell thickness and ligand type for the rapid, efficient separation of larger molecules such as intact proteins and other biomacromolecules up to at least 400 kDa.
This study examines the impact of ECD from a Waters Alliance 2695 system on the performance of 2.7 μm HALO® C18 Fused-Core superficially porous particle columns of various dimensions.
The development of these new wide-pore fused-core particles now allows the HPLC separation of a wide range of molecules of different sizes with advantages of the SPP configuration.
To meet this need, larger pore superficially porous particles with appropriate surface properties (Fused-Core® particles) have been developed with a pore size of 400 Å, allowing large molecules (<500 kDa) unrestricted access to the bonded phase.
A study of the shell thickness of the new fused-core particles suggests a compromise between a short diffusion path and high efficiency versus adequate retention and mass load tolerance.
Traditional precipitation of BSA by adding an equal volume of organic solvent, often successfully used with conventional HPLC-PDA, was found insufficiently robust when novel fused-core HPLC and/or UPLC-MS methods were used.
