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We are also investigating ways to fuse modules to optimize execution times.
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In that example, when the fused NRPS module was excised from the megasynthetases, the PKS module was able to synthesize a polyketide product that is longer than that present in preaspyridone (pentaketide instead of tetraketide).
For instance a protein from Actinobacterium Symbiobacterium thermophilum (GI: 51893458) contains the N-terminally fused LuxR module, comprising the wHTH domain and an uncharacterized domain annotated as COG3860.
Searches show that C-terminal D5-like helicase modules fused with the TV-Pol domain are closest to those fused to the prim-pol family of AEPs.
Over time, modules fused at their points of contact and as they contracted, decreased the internal void space, which reduced the overall perfusion through the construct.
P450 BM3 and the nitric oxide synthases are related classes of flavocytochrome mono-oxygenase enzymes, containing NADPH-dependent FMN-containingoxidoreductaseoreductase modules fused to heme b-containing oxygenase domains.
In general additional modules fused to a catalytic domain may play a role in substrate specificity by providing a specific binding site or shaping the active site to recognize a substrate with a different shape or size [40].
As described above, AA8 is frequently associated to AA3_1, but AA8 can also be found fused to distinct modules.
We validated the ability of the scaffoldin to co-localize two different dockerin-containing proteins by using a reporter system composed of fluorescent proteins fused to dockerin modules.
Linkers and protein modules fused to the linkers, were at 5 μM, and were incubated 1 h at 24 °C with a 1.5-fold excess of relevant staple.
Yet another remarkable cluster (SMC14) on the chromosome has one of its putative NRPS modules fused to a β-lactamase domain (PFAM number PF00144).
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