Applying the generalised missing-indicator approach suggested by Dardanoni et al. (2011) we, furthermore, checked for potential bias induced by improper imputations.
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They were then verified and completed by one reviewer (LAF), and furthermore checked by another reviewer (MA-L) for accuracy.
The genome composition of hybrid adults and offspring was thus determined independently four times and furthermore checked for agreement with the remaining, less informative, loci.
Therefore, in the present study, we used not only H.E but also immunohistochemical staining CAM5.2, furthermore checked by AE1/AE3, to make a more accurate diagnosis of tumour budding and D2-40 to detect lymphatic involvement.
We immunohistochemically examined tumour budding at the deepest invasive margin of 56 surgically resected submucosal colorectal carcinomas using anticytokeratin antibody CAM5.2, furthermore checked by AE1/AE3, and determined the relation between tumour budding and clinicopathological factors.
Furthermore, after checking for conservation in the closely related species Leishmania major, only 5 composites were considered genuine.
Furthermore, by checking the expression differences after stress treatments in callus, we also observed that part of the LSGs expressed responsive to a variety of abiotic stresses.
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