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The cells were furthermore amplified during 5 passages for preparing passage 33 (P33) samples for omics analysis.
Furthermore, amplified genes often act as markers of tumour behaviour, drug response, patient outcome, and may represent targets for future molecular cancer therapy.
We identified each of the five cytosolic MVA genes/cDNAs in the genome of the rhodophyte Galdieria sulphuraria and, furthermore, amplified four of them from the glaucophyte Cyanophora paradoxa.
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Furthermore, we amplified the number of colonies by a factor of about 10 in order to maintain the complexity of the libraries throughout the subsequent cloning steps (Table 1).
Furthermore, we amplified single bands in 18 markers (~6%), from a phylogenetically distant clade from the genome resources, L. catta.
Furthermore, RPA amplified libraries showed relatively high levels of chimeric and duplicate reads (see additional file 1, Table S2).
Furthermore, we amplified and sequenced 33 cell lines (including 12 lung cell lines and six xenografts) and 46 clinical samples (RNA extracted from 23 matched lung tumour and normal tissue) from patients treated at our institution and did not identify any of the three mutations reported by Davies et al (2005).
Furthermore, FGFR1 is amplified in 10% of human breast tumours and has been linked to a poor response to treatment in breast cancer patients [ 17].
Furthermore, ITS pseudogenes were amplified with specific primers from 10 of the 28 strains tested, including eight single-ascospore and two tissue isolates.
Furthermore, displacements can be amplified by anelastic effects in the hanging wall near the trench axis.
Furthermore, the PCR fragments amplified from the tagged BoNT-encoding plasmids were sequenced and it was confirmed that the introns had inserted correctly into the chosen target sites within the neurotoxin genes in all three plasmids.
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