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A further washing step was done with buffer to remove excess of sample and to prepare the microarray for 1 h incubation with a labeled Alexa Fluor mix.
After further washing, cells were transferred for confocal microscopy.
After a further washing step with ethanol, the protein pellet was dried in a vacuum centrifuge.
The final step was the application of a weak solution of acrylic polymer to consolidate and protect the surface, and further washing.
After further washing, immunoreactive signals were determined by chemiluminescence.
After further washing, the sections were fixed with mowiol.
After further washing steps cells were resuspended in FACS-buffer before analyses.
After further washing, the cultures were incubated in a solution containing a mixture of the secondary antibodies.
These hearts were then extracted and underwent retrograde perfusion with heparinized saline prior to sectioning and further washing.
After further washing in TBST, the immunoblots were developed with the ECL system (Perkin Elmer, USA) following the manufacturer's instructions.
After three further washing steps filters were developed using the ECL™ Western Blotting Detection Kit (Amersham Biosciences).
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