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More importantly, compared to the SYS model, the ST model was further validated by the detection of alpha-synuclein expression and the ultrastructural changes in SNc.
This finding was further validated by assessing the expression of the axon guidance molecules neuritin (NRN1) and roundabout receptor ROBO1 and -2.
The above results were further validated by determining the expression of putative markers for each infection in key tissues using real-time PCR.
That these results were specific for the genes concerned and not due to any off target effect were further validated by the survival kinetics of ilvB antisense expression.
The C79I or C79F mutants were further validated by qRT-PCR for expression of IFNβ and IL-6 in IMR32 and HEK293T cells.
Reciprocally, these findings were further validated by knocking down the expression of endogenous miR-29b by cotransfecting cells with anti-miR-29b.
These results should however be further validated by testing SlGGP1 expression in fruits of a larger collection of tomato cultivars with a wider range of totAsA-AsA concentrations.
These patterns were further validated by independent expression analyses (Supplementary Fig S5C and D).
These findings were further validated by silencing PTEN expression in the gastrulating chick embryo.
This association of CD8+ PD-1 and PD-L1 expression with HLA-DR expression was further validated by reciprocal analysis of HLA-DR expression on CD14 monocytes.
Involvement of ITPKA and PNCK in TCDD-mediated CYP1A1 up-regulation was further validated by screening-independent expression knock-down.
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