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At 254 days, the specimens stored in saturated lime solutions were cut to 100 mm in length and further vacuum saturated (− 20 in-Hg) with deionized water for at least 1 h.
For O2 – staining detached leaves were first infiltrated with K2PO4 buffer (pH 7.8) for 30 min and then the buffer was changed into 0.1% NBT (Nitroblue tetrazolium, Sigma-Aldrich) in 10 mM K2PO4 buffer and the leaves were further vacuum infiltrated for 30 min.
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Further, vacuum-assisted closure therapy draws wounds closed by applying controlled, negative pressure while smoothly removing infectious material and interstitial fluids, thus allowing tissue decompression [ 12].
By two-step pyrolysis and further high vacuum sublimation of the soot the fullerenes C60 and C70 were obtained in extractable amounts.
The international community owes it to Libya and stands to gain from helping the Libyans and investing in preventing further power vacuum and collapse.
The upper filtration liquid was further concentrated using vacuum evaporator and subsequently using vacuum freeze drying to obtain powdery production of lipopeptides for further purification.
Further, if the vacuum has energy, the question arises concerning the origin of the vacuum and its energy.
Further to the vacuum casting, to create an electrically conductive layer on the vacuum-casted electrode, an Ag/AgCl electroless deposition method has been developed.
The metal is further purified by vacuum distillation.
But each day that he accelerates his pursuit of Madoff's collaborators, he steps further into the vacuum of leadership left by others, including the Obama administration's Department of Justice.
The monomer was further purified by vacuum distillation over CaH2.
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