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At pH 5.5, the T domain further unfolds, resulting in the exposure of its initially buried C-terminal hydrophobic helices (TH8 9) and subsequent oligomerization.
The strong increase in deuterium uptake in TH 1 4 suggests the N-terminal region further unfolds and releases the initially buried hydrophobic C-terminal TH8 9.
The HSP104 domains that contact the aggregate undergo an ATP-driven conformational change that separates or further unfolds the misfolded aggregates and then releases them.
At pH 5.5, the transition is complete, and the protein further unfolds, resulting in the exposure of its C-terminal hydrophobic TH8 9, leading to subsequent aggregation in the absence of membranes.
At pH 5.5, the T domain further unfolds to expose the C-terminal hydrophobic hairpin (TH 8, residues 333 350; TH 9, residues 360 367) and the interhelical loop (TL 8 9), providing a hydrophobic surface for subsequent oligomerization.
In this way, we are able to demonstrate that, at pH 5.5, the T domain further unfolds, allowing the C-terminal hydrophobic hairpin (TH 8, residues 333 350; TH 9, residues 360 367) and the interhelical loop TL8 9 (residues 351 360) to be exposed to aqueous environment, creating a hydrophobic interface for T domain aggregation in the absence of membranes.
Similar(5)
Those two matches may be all that separates their legacies, which will further unfold here.
The specialized field now called "design" -- as opposed to, say, cabinetmaking, textile weaving or blacksmithing -- represents the further unfolding of the path they pioneered.
And the future of New York architecture may well represent the further unfolding of southern routes from the Old World to the New.
"The positive impact of Olympic effects on the UK market pale into insignificance alongside macro events such as the further unfolding of the eurozone debt crisis and the outlook for global growth in 2012," he said.
Further, unfolding the interesting structural and functional aspects of these enzymes in the last few years has qualified them as a potential drug target against various diseases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com