Sentence examples for further transformed with from inspiring English sources

These pTUM4 transformants were further transformed with pASK-IBA32 plasmid encoding the Bj-xtrIT(His6) gene and plated on agar with 100 μg/ml AMP plus 25 μg/ml CAM overnight at 37 °C.

Competent E. coli BL21 DE3) cells (Invitrogen, Carlsbad CA), which have isopropythio-β-D-galactoside (IPTG -inducible T7 RNA polymerase expressIPTG -inducibleformed wiT7 pJL100 oRNAJL200; some of these transformants were made compolymerase furthexpressionrmed were pJL300.

Transformation of A. thaliana with the first two genes consolidated on a single binary vector yielded transformants producing high levels of DGLA, and these plants were further transformed with mouse prostaglandin H synthase (PGH) genes to produce prostaglandins.

The torenia, whose F3′,5′H expression had been suppressed, was further transformed with the F3′H gene driven by a constitutive promoter.

For construction of defined double mutants, V. cholerae strains carrying a deletion mutation was further transformed with genomic DNA fragments of appropriate strains (carrying TnFGL3 inserthe2 in the presence of chitin by using previously described methods26,36.

TMB 4440 was further transformed with the constructed linearized YIpAGS2 that carries C. necator PHB pathway gene or with a linearized integrative control vector (YIplac128), generating strains TMB 4443 (PHB+) and TMB 4444 (PHB-), respectively.

Some derivatives were further transformed either with the simian virus 40 (SV40) large T antigen, termed HEK293T, or with the Epstein Barr virus (EBV) nuclear antigen 1 (EBNA1), termed HEK293E, in order to mediate semi-stable episomal propagation of vectors containing an origin of replication (ori) of SV40 or EBV, respectively.

The self-adjointness and the orthogonality condition for the eigenfunctions of the further transformed system with elastic boundary conditions are derived.

Furthermore, these could not be further transformed using telesubstitution with ammonia, to give 8-amino imidazo[1,2- a]pyrazines as planned.

Verbal autopsies (VAs) were conducted using the 2007 World Health Organization (WHO) standard VA questionnaires, and VA data further transformed to align with the 2012 WHO VA instrument.

The VA data were further transformed to align with the WHO 2012 VA standard format and were then processed using the InterVA-4 model to assign COD.