The compounds which were found satisfactory by the above two methods at a maximum concentration of 50 µg/ml were diluted further to concentrations viz., 25, 12.5, 6.25, 3.125 and 1.56 µg/ml respectively.
Each of the synthesized derivatives was dissolved in DMSO to obtain a concentration of 50 µg/ml and diluted further to a concentration of 25 and 12.5 µg/ml.
The samples may need to be diluted further to same concentration with appropriate volume of membrane protein dissolution buffer in the kit.
The extract was further subjected to concentration using a rotary evaporator (Buchi, India) under reduced pressure.
Beans were the legumes that showed the highest levels of enzyme inhibitors and lectins, however processing such as cooking and dehydration significantly reduced (p < 0.05) their levels further to negligible concentrations.
Recombinant mouse fibroblast growth factor-8b carrier-free (FGF8b, R&D Systems, Minneapolis, MN) was reconstituted in sterile phosphate buffered saline (PBS) and diluted further to final concentrations.
All drugs were diluted further to an appropriate concentration in growth media before use for minimum inhibitory concentration (MIC) determination and for their effects on sporulation.
This expansion can be accomplished by diluting each of the pools further to establish other concentration ranges, prior to introducing them into the background human total RNA.
Increasing the PEO concentration further to 70% (molar fraction PEO) leads to a high PEO concentration regime where PEO spherulites become readily apparent by optical microscopy.
Upon increasing the salt concentration further, to 2 M NaCl, NP bound with ssDNA molecules start aggregating.
