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A further technical limitation was with the ultrasound machine used in the study which initially did not have an M-mode or video capture capability.
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Further, technical limitations of sublingual videomicroscopy have to be recognized.
We could not further investigate the technical limitation that made the uncalibrated estimation of CI inaccurate, because we could not analyze all the components of the proprietary algorithm.
It was further confounded by the technical limitation that whole-mount sensitivity is typically lower than that achieved using immunohistochemistry (Couffinhal et al., 1997; Pereira et al., 2006) or RNA hybridisation techniques.
Described herein is a high-throughput method for measuring all three key metabolic parameters under defined [O2] thereby further addressing such technical limitations.
Moreover, a number of limitations to visual proteomics might be overcome by further technical developments.
Unfortunately, we are unable to further narrow the brain regions or neuronal types involved in this phenotype due to the technical limitation that there are not selective RIM1 antibodies that work for IHC.
Yet, there are limitations to these technologies, as discussed below, some of which could be addressed through further technical developments.
Due to this technical limitation, the oxygen concentrations shown in the y-axis labels were somewhat arbitrary– 1(f)) and further analysis, such as calculation of oxygen consumption rates, was therefore not attempted.
"That's a technical limitation".
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CEO of Professional Science Editing for Scientists @ prosciediting.com