Thus, for instance, a group of proteins classified as functionally related on the basis of a similar catalytic activity, might be further sub clustered if the similarities between other 3D patterns are considered.
Among the large P.melalophos cluster, one further sub-cluster is indicated, which includes P.m.melalophos from Bengkulu (population 7) and the southern Sumatran P.m.mitrata (populations 4-6).
There were distinctly different expression profiles between the tumor group and the control mucosa group, and the histological subsets of mixed type, diffuse type and intestinal type cancer demonstrated further sub-clustering.
Under each of these main clusters, genotypes were grouped further into sub clusters.
Cluster 1 can be further sub-classified into three clusters (cluster 1-a, 1-b, and 1-c) by its location and components.
Each major cluster can be further sub-divided into 2 sub-clusters: sub-clusters 1a and 1b (n = 13 and n = 7 samples respectively; mean beta values 0.14 and 0.34 respectively) and sub-clusters 2a and 2b (n = 12 samples in each cluster; mean beta values 0.50 and 0.69 respectively) (p < 0.001; ANOVA).
Genes within each GO category group were further sub-grouped into clusters of genes with similar expression profiles using K-means clustering of previously published expression data for conidial germination [ 10].
While evaluating the similarity matrix based on the Dice coefficient for all nuclear specific RAGEP markers and whilst constructing a UPGMA tree, it was observed that the various population groups of H. puera fall in two clusters, which are further divided into two major sub clusters.
Using more automated approaches, ProtoNet (Rappoport et al., 2014) provides an automatic classification of similar proteins which are further sub-classified into clusters using an information-theoretic protocol based on available annotations.
Further eight sub-clusters contained co-transcribed and functionally tightly associated genes.
In Cluster I, 11.3% (8) of total genes were grouped and further sub divided into two groups with each sharing 2.8% (2) and 8.5% (6) genes, respectively.
