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Cells are pelleted by centrifugation and all further steps are performed by a robot system.
Two further steps are performed by a single protein, BAG-1, another co-chaperone of Hsp70.
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Further steps were performed as described above.
Further steps were performed as described in [23].
All further steps were performed at RT in a hydrated chamber.
All further steps were performed at 0 4°C, and all buffers contained 5 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 10 mM sodium metabisulfite, and 2 ug/mL leupeptin.
All further steps were performed at room temperature.
Further steps were performed as previously described [20].
All further steps were performed with reagents from Life Technologies Darmstadtt, Germany).
All further steps were performed on ice and all centrifugation steps at 4°C.
Treated cells were washed with ice-cold PBS, and all further steps were performed on ice.
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