Further site-directed mutagenesis analyses demonstrated that the FadR mutants (F74G and/or W75G) do not exhibit the detected DNA-binding activity, validating above structural reasoning.
Further site-directed mutagenesis and functional complementation identified 14 highly conserved surface-exposed residues, as well as additional residues involved in TraC dimerization [186].
Plans to use crowd-sourcing to map further sites are being explored.
Joan Edwards, at the Wildlife Trusts, said: "When will further sites be designated?
Luxury buses will take passengers on to further sites, and afternoons will be dedicated to exploring stops on foot.
The new site will be operated by Veolia, which currently owns seven operational incinerators, which they call "energy recovery facilities", with a further site under construction in Staffordshire.
The UK's biggest online grocer opened two dark stores in 2013 and has previously said it is scouting for further sites in Birmingham and Manchester.
In the next few months two further sites, including Leeds, will switch to the system, which promises at least 40 hours a month.
Back at the airfield, Brigadier Alex Birtwhistle, who is in charge of the operation, said the army was looking at five further sites in Cumbria for the mass burial.
Further sites, not formally confirmed but likely to be on the list, include South Kensington, Bermondsey and Kidbrooke stations and property close to Oxford Circus.
Already a player on every continent apart from Africa, the company wants to open a minimum of 50 further sites globally in 2012.
