Apps are divided into subsets for iPhone or iPad and are further separated into groups for the most popular free apps, the most popular paid apps and the highest grossing apps of the year.
We further separated the analyses for set A and set B since they included different question items and were answered by different students.
Time spent waiting before consultation has attracted much research attention, and can be further separated into waiting time for registration, and waiting time for consultation [ 6].
All animals were kept in the same cages until the reintroduction took place, excluding those cages containing large numbers of individuals (five plus) which were further separated out in Oxford for welfare and logistical reasons – see Study design for details.
Soluble and membrane proteins were further separated by ultracentrifugation (20,000×g for 45 min at 4°C). 10 µg of total protein extracts were treated with β-Mercaptoethanol (14.4 mM) and SDS (2%) containing loading buffer and heated at 100°C for 5 minutes.
The proteins were transferred to PVDF membranes in a transfer buffer [25 mM Tris HCl buffer (pH 7.4) containing 192 mM glycine and 20% v/v methanol] and further separated at 400 mA for 2 h at 4°C.
For the isolation of the crude membrane fraction of mouse brain, brains from the C57BL/6J mice were homogenized in a buffer containing 250 mM sucrose, 3 mM imidazole, 1 mM EDTA and protease inhibitors and were centrifuged at 1,000×g for 10 min. The supernatant was further separated by ultracentrifugation at 100,000×g for 1 h.
Blood samples (300 μl) were collected from mice tail veins on the specified days, in the presence of heparin, and the plasma was further separated by centrifugation (1200 r.p.m. for 10 min).
MACS-sorted cells were further separated by FACS sorting and cultured for 4 weeks in the presence of IL-2.
The solution was further separated by centrifugation at 10,000 rpm for 10 minutes and the supernatant (1.5 mL) was transferred into a vial and analyzed using Gas chromatography mass spectrometry following the method of Hema et al. [ 22].
