Sentence examples for further round of selection from inspiring English sources
To achieve moderate levels of mutant ank expression in ATDC5 cells that were comparable with expression of the endogenous transcript, we chose to subject our transduced cells to a further round of selection using limiting dilution.
Further rounds of selection were used to enrich for P8 variants that supported higher levels of C-terminal peptide display.
The aptamers that bound to L. monocytogenes were isolated using centrifugation, PCR amplified, and purified to yield an enriched ssDNA pool suitable for further rounds of selection.
Phage from these stocks were amplified for further rounds of selection.
Following 24 h of recovery and a further 48 h of puromycin selection (1 μg/ml) the transfection was repeated and cells subjected to a further round of puromycin selection to enrich for transfectants.
B cells continue their development in the spleen, where they pass through transitional stages transitional type 1 (T1) and transitional type 2 (T2) and are subjected to a further round of negative selection before becoming totally mature.
Here, a strategy combining one further round of backcrossing, two to four selfings and marker-assisted selection with simple sequence repeat (SSR) markers was applied.
In a separate approach, we performed 2 further rounds of ex vivo selection with libraries pre-selected for 2 rounds on PymT cells in vitro (like in Figure 1, pathway A).
Likewise, the F3 and F4 generation was produced by two further rounds of selfing and PCR selection.
By further rounds of random mutagenesis and ribosome display selection, binders with picomolar affinity were obtained that were entirely monomeric and could be expressed at high yields in the cytoplasm of Escherichia coli.
