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2179 sequences were further retrieved from these protein files.
The FASTA formats of the sequences were further retrieved for analysis.
Additional potential relevant articles were further retrieved through a manual search of references from original reports.
The internal coding regions were further retrieved from different PwRn1 copies (35 copies [GenBank: EU622539 – EU622573], Avg. divergence = 0.051 ± 0.002).
We further retrieved experimentally verified CRMs from the REDfly database and other literatures to distinguish putative and known CRMs.
For validating the signatures obtained from analyzing the primary dataset, we further retrieved 15,785 human or avian influenza A viral protein sequences from NCBI's Influenza Virus Resources.
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Further, retrieving the first instance during the presentation of the second allows the learner to compare across the instances and create generalized knowledge across the episodes.
After that, the seed file was used to further retrieve the whole genome database of G. raimondii (Paterson et al. 2012) by HMMER 3.0 software (Finn et al. 2011).
Further retrieving genome bins of Thermotogales might help to resolve the possibility for the association.
It makes use of the protein information retrieved by the first service to further retrieve the related 3D structure information in the form of Protein Data Bank identifiers.
Thereafter, we used Perl scripts to further retrieve the accessory genome of Hif KR494 using different parameters, that is, genes absent from (i) all H. influenzae reference genomes used in the present study (Table 2), (ii) related Haemophilis spp. reference genomes (Table 2) or (iii) genes not found in all H. influenzae genome sequences available in the current databases.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com