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Interestingly, further residue mutant experiments showed that a single basic residue in the filter region determined the insensitivity of KCNQ1 channels toward scorpion toxins.
The residue name is randomly placed on a circle with the centre being the centroid of the residue which is the arithmetic mean position of all the residue atoms and a radius of M a x(d i s t a n c e(c e n t r o i d,i))+0.5 with i the residue atoms, i.e. the distance between the centroid and the further residue atom plus 0.5 Å.
Further residue changes occur at positions relevant for intranucelosomal interactions.
Of the eight residues we mutated, two resulted in altered drug export function (P485A and M549A) and a further residue (I573A) perturbs the trafficking and maturation of ABCG2 glycosylation.
A further residue that when mutated gave a strong effect on planar polarity but not on canonical activity (E279) protrudes into the deep central pocket in our model and thus might be part of a specific binding surface that interacts directly with Dsh only when the Fz receptor adopts a certain conformation.
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From (eps)ad, (eps)ox and analysis of oxidation products a mechanism is postulated which consists of an adsorption with simultaneous dehydrogenation step followed by cycle rupture and electroadsorption and dehydrogenation of further residues.
Further residue-based decomposition of the protein electric field (−15.4 MV/cm) showed that there is a strong degree of overlap between the residues that contribute significantly to the field along the D A axis and the network of coupled motions that has been identified for DHFR catalysis.
Further, residues of antibiotics may still be present in the meat of treated animals [ 29, 12].
CGRP derivatives were substituted with either cysteine or alanine; further residues were introduced at position 6.
Next, we modified 6 further residues near T118; of these, E123 cross-linked with CpoB.
Mutations of two further residues in this cluster, Thr102 and Thr108, have been shown elsewhere to lose both functions [ 22].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com