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Taken all these limitations into account, it is critical to stress that this is a pilot study that needs further replication and validation as finding biomarkers from the identified differentiating significant compounds still requires optimization of target-specific analytical methods and validation of these methods with their reference materials and proficiency tests [50].
These novel findings require further replication and validation.
Sensitivity and specificity analysis suggest the IPAT could provide a way of allowing staff to assess psychological distress among critical care patients after further replication and validation.
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Therefore, although there are strongly significant evidences that component genes from the 7 gene sets are associated with diabetes traits, these 25 common genes are only susceptibility candidate genes for diabetes pathogenesis, which requires further replications and experiment validations in the future study.
These findings merit further replication and, if confirmed, warrant fine mapping and functional validation studies.
However, further replication and functional studies are still required.
However, due to the small size of the "inflammatory" COPD group, further prospective replication and validation is needed.
These results provide a wealth of new information on potential candidate genes that will require further exploration, replication and validation in future studies.
Further work will be necessary for external replication and validation of these results.
Our objective will be addressed in aims 1) identification of miRNA and proteomic signature in sALS and fALS progression; and 2) replication and validation of the identified hits.
Replication and validation cohorts in diagnostic studies are essential.
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