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Further replicates are required to provide greater confidence of the estimated LD99 for A. ferus eggs.
Given the outliers present at doses of 40 to 100 Gy in the third replicate, it is necessary to conduct further replicates in the future to provide greater confidence in the LD estimates presented here.
This result further replicates previous work from our laboratory [38] and confirms the reliability of our decoding method.
With further replicates, this type of information could be used to determine if sharks are grouping or avoiding particular individuals within the population, based on a number of phenotypic traits such as sex, size and species.
Further replicates that go beyond the sampling strategy defined by DIN 38414 are needed to be able to fully comprehend the toxicity and associated effects of samples used in this study.
Although variability in this system could be explored in more detail with further replicates of the time series, we feel that we are able to reach sound conclusions regarding the temporal organisation of gene activity around the MBT based on the data we have described.
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The inability to further replicate is termed replicative senescence and can be induced by a plethora of factors, such as short and uncapped telomeres, oxidative stress, and mitochondrial DNA damage (Fig. 2) (Chen et al., 2007).
The master mould was further replicated by PDMS.
The results in the controls were further replicated in 10.8% of an independent series of 102 non-cancer controls.
The phenomenon, what has come to be known as the rubber hand illusion (RHI), has since been further replicated, by various techniques, multiple times (for reviews, see Ehrsson, 2009, 2012; Makin, Holmes, & Ehrsson, 2008; Tsakiris et al, 2010; Tsakiris 2011), even in non-humans (Wada, Takano, Ora, Ide, & Kansaku, 2016).
And through an innovative public-private partnership, earlier this month the CAS-Carrera program was awarded a $3.5 million grant from the Edna McConnell Clark Foundation and the Obama administration's Social Innovation Fund (SIF) to deepen the program's impact in New York City and further replicate it nationally.
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