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Such values may require further refinement using trial-and-error procedures upon implementation.
After several rounds, 200 structures were calculated and 100 structures with lowest target function were selected for further refinement using the AMBER program (version 7.0) [59].
Using a whole-genome scan employing 287 single-nucleotide polymorphic (SNP) markers and further refinement using six additional SNP markers and four microsatellite markers, the causative mutation was mapped to a 5.3-cM region on chromosome 4.
However, these values need further refinement using additional samplings.
Further refinement using the exclusion and inclusion criteria and duplicate exclusion resulted in 47 studies identified and reviewed (table 1).
Further refinement using manual checking by experts of the 180,000 names assigned by the software produced an estimated final sensitivity of 97%.
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A further refinement uses urban indexes to estimate the green needs of each selected area.
As such, it is an improvement on current methods and lays the basis for further refinements using more extensive datasets, such as community based diagnoses.
For further refinement use the Advanced Keyword Search to search for the subject keywords [geology place name maps] (e.g., geology tokyo japan maps).
The signal subtracted particles were used for further 3D refinement using local search by restricting sigma_ang to 5°, using the map of SUR1 ABC transporter module as the starting model.
Further refinement continued using a Delphi process, where the draft was emailed between the two groups until a consensus was reached.
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