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A further readout for upregulated JNK activity is increased expression of Matrix Metalloproteinase-1 (MMP1).
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Since LKB1 is likely to be the major upstream kinase for LKB1 in islets (I. Leclerc and G. A. Rutter, unpublished results), AMPK activities were also measured as a further readout of LKB1 activity.
I don't have any further readout on what the president thought was a constructive meeting.
Moreover, late-onset or incomplete penetration of the disease phenotype can complicate further readout and genotypic correlation.
We therefore performed time course experiments and tested for cleaved PARP as readout for apoptosis to further substantiate our initial finding with a different assay at different time points.
Having implicated endotrophin as a critical mediator of cisplatin resistance, we wanted to further substantiate the endotrophin-induced EMT as a downstream readout for cisplatin resistance acquisition by utilizing a histological approach.
Our studies also open the door to using changes in metabolite diversity as readout for cumulative damage in age-related studies, and further improvement in sensitivity of this technology should increase the fraction of damage over functional metabolites.
We further observed that stimulation with ATP induced the release of β-hexosaminidase, a common readout for degranulation (not shown).
In anticipation of further studies with other epigenetic agents to induce iTreg cells, we desired to have a rapid readout for our suppressor cell assay.
In order to explore whether this readout for convergence is unique to ERK1/2 or is shared with other MAPKs, we further analyzed possible convergent activation of p38 and JNK.
To demonstrate further that that CLN3 overexpression does indeed inhibit Notch signalling, transcription of the cut gene was used as readout for the Notch pathway.
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