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The extracted information was populated to a domain ontology for further querying through a web service.
Sequences classified in each analysis as belonging to a reference were withdrawn from further querying since they do not represent 'novel' reads.
It is also possible to export all results either as a list for further querying within YeastMine, as a table to the Galaxy tool (7), or as a file to your desktop.
Further querying of ShewCyc reveals that HexR can repress not only pykA but also enzymes of the Entner Doudoroff (ED) pathway transcribed from the four gene operon (zwf-eda-edd-pgl), which is located upstream of the hexR, but on the opposite strand.
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Further queries to OSF resulted in the following guidelines: "cultural, sociological, political issues, and of course the human story" – in other words, anything.
We further queried KEGG for specific pathways affected.
The focal genes are further queried for their SNPs using ENSEMBL [9] and linkage disequilibrium information from HapMap [10].
Given the importance of p53 mutations in cancer susceptibility and progression, we further queried central SNPs for the p53 focal genes.
In order to ensure that creations were not simply extinctions in a majority of the species studied here, we further queried the other eukaryotic genomes present in Ensembl v.41.
This study provides not only a genome-wide view of the effect each gene deletion has on peroxisome morphology, but also provides an extensive framework from which to further query the complex array of processes that regulate the response of cells to fatty acids and the biogenesis of peroxisomes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com